human il 9 duoset elisa r d system (R&D Systems)
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human il 9 duoset elisa r d system
Human Il 9 Duoset Elisa R D System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+9+duoset+elisa+r+d+system/Human+IL-9+DuoSet+ELISA/10__1016_slash_j__celrep__2024__115136-206-121-125
Average 93 stars, based on 8 article reviews
Human Il 9 Duoset Elisa R D System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+9+duoset+elisa+r+d+system/Human+IL-9+DuoSet+ELISA/10__1016_slash_j__celrep__2024__115136-206-121-125
Average 93 stars, based on 8 article reviews
human il 9 duoset elisa r d system - by Bioz Stars,
2026-11
93/100 stars
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1) Product Images from "Mechanical force receptor Piezo1 regulates TH9 cell differentiation"
Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation
Journal: Cell Reports
doi: 10.1016/j.celrep.2024.115136
Figure Legend Snippet: Figure 6. Piezo1 deficiency inhibits TH9 cell differentiation through the SIRT3-SDHA-OXPHOS metabolism pathway (A) The oxygen consumption rate (OCR) was examined as a readout for OXPHOS in CD4+ T cells isolated from WT and Piezo1/ mice for 5 days under TH9 cell- inducing conditions. (B) Mean fluorescent intensity (MFI) of SDHA in CD4+ T cells isolated from WT and Piezo1/ mice for 5 days under TH9 cell-inducing conditions. (C) SDH/complex II activities of T cells isolated from WT and Piezo1/ mice for 5 days under TH9 cell-inducing conditions by ELISA. (D) Mass spectrometry analysis of the metabolite abundance of fumarate (FA), succinate (SC), and malate (MA) in CD4+ T cells isolated from WT and Piezo1/
Techniques Used: Cell Differentiation, Isolation, Enzyme-linked Immunosorbent Assay, Mass Spectrometry
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REAGENT or RESOURCE SOURCE IDENTIFIER GsMTx4 MedChemExpress, USA Cat#HY-P1410 Ionomycin Sigma-Aldrich, USA Cat#I0634 Yoda1 MedChemExpress, USA Cat#HY-18723 Critical commercial assays Co-IP buffer Thermo Fisher Scientific, USA Cat#88804 CD4+ T cell isolation kit Miltenyi Biotec, USA Cat#130-104-453 ECL kit Beyotime Biotechnology, China Cat#P10018AS Enhanced BCA protein assay kit Beyotime Biotechnology, China Cat#P0009 Foxp3/transcription factor staining buffer set BD Bioscience, USA Cat#560133 Mito stress test kit Seahorse Bioscience, USA Cat#101706-100 NE-PER nuclear and cytoplasmic extraction reagents Thermo Fisher Scientific, USA Cat#78835 RIPA lysis buffer Beyotime Biotechnology, China Cat#P0013B RNeasy mini kit Qiagen, Germany Cat#74106 PrimeScriptTM RT reagent kit TakaRa, Japan Cat#TakaRa_RR037A SuperReal preMix plus SYBR Green Tiangen, Germany Cat#FP205-02 Dual-Luciferase assay system Promega, USA Cat#E1910 Mouse IL-9 DuoSet ELISA R&D system, USA Cat Lysis:Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Aluminum hydroxide Sigma-Aldrich, USA Cat#239186 Antimycin A Sigma-Aldrich, USA Cat#A8674 Bovine serum albumin (BSA) Sigma-Aldrich, USA Cat#A7030 CaCl2 Merck, Germany Cat#C5670 Collagenase D Worthington, USA Cat#LS005273 CoCl2 Merck, Germany Cat#409332 Deoxyribonuclease I Beyotime Biotechnology, China Cat#D7076 DMEM Thermo Fisher Scientific, USA Cat#C11995500BT DMSO Sigma-Aldrich, USA Cat# D2650 Dooku1 MedChemExpress, USA Cat#HY-126010 Dimethyl malonate (DMM) Selleck, USA Cat#S6208 Fetal bovine serum (FBS) Every Green, Belgium Cat#11011-8611 Fibronectin Sigma-Aldrich, USA Cat#FG001 Fura-2 a.m. Sigma-Aldrich, USA Cat#F1201 Glucose Invitrogen, USA Cat#15023021 HEPES Invitrogen, USA Cat#15630130 Hoechest 33342 Beyotime Biotechnology, China Cat#C1029 KCl Thermo Fisher Scientific, USA Cat#ECCON84BT L-glutamine Sigma-Aldrich, USA Cat#G8540 Lipofectamine 3000 Thermo Fisher Scientific, USA Cat#L3000150 Methacholine Sigma-Aldrich, USA Cat#62-51-1 MgCl2 Invitrogen, USA Cat#AM9530G NaN3 Sigma-Aldrich, USA Cat#S2002 NaCl Invitrogen, USA Cat# AM9760G NaOH Merch, Germany Cat#1.09137 4% paraformaldehyde (PFA) Solarbio, China Cat#P1110 Oligomycin Sigma-Aldrich, USA Cat#495455 Ovalbumin (OVA) Sigma-Aldrich, USA Cat#S7951 Percoll GE Health, USA Cat#17-0891-01 Phorbol ester (PMA) Sigma-Aldrich, USA Cat#P8139 Protein A/G plus agarose beads Thermo Fisher Scientific, USA Cat#26159 RPMI1640 Thermo Fisher Scientific, USA Cat#C11875500BT Rotenone Sigma-Aldrich, USA Cat#R8875 Recombinant human IL-2 protein R&D system, USA Cat# BT-002 Recombinant human TGFb1 protein R&D system, USA Cat#7754-BH Recombinant human TGFb1 protein Peprotech, USA Cat#PHG9204 Recombinant mouse IL-4 protein R&D system, USA Cat#404-ML Recombinant mouse IL-4 protein PeproTech, USA Cat#214-14-5UG Recombinant mouse IL-6 protein R&D system, USA Cat#406-ML Recombinant mouse IL-12 protein Peprotech, USA Cat# 210–12 Recombinant mouse IL-23 protein R&D system, USA Cat#11269-ML SDS-PAGE sample loading buffer Beyotime Biotechnology, China Cat#P0015L Sodium pyruvate Sigma-Aldrich, USA Cat#S8636 TRI reagent Sigma-Aldrich, USA Cat#T9424 3-TYP MedChemExpress, USA Cat#HY-108331 Triton X-100 Sigma-Aldrich, USA Cat#S100PC Glucose Sigma-Aldrich, USA Cat#40163 (Continued on next page) 16 Cell Reports 44, 115136, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER GsMTx4 MedChemExpress, USA Cat#HY-P1410 Ionomycin Sigma-Aldrich, USA Cat#I0634 Yoda1 MedChemExpress, USA Cat#HY-18723 Critical commercial assays Co-IP buffer Thermo Fisher Scientific, USA Cat#88804 CD4+ T cell isolation kit Miltenyi Biotec, USA Cat#130-104-453 ECL kit Beyotime Biotechnology, China Cat#P10018AS Enhanced BCA protein assay kit Beyotime Biotechnology, China Cat#P0009 Foxp3/transcription factor staining buffer set BD Bioscience, USA Cat#560133 Mito stress test kit Seahorse Bioscience, USA Cat#101706-100 NE-PER nuclear and cytoplasmic extraction reagents Thermo Fisher Scientific, USA Cat#78835 RIPA lysis buffer Beyotime Biotechnology, China Cat#P0013B RNeasy mini kit Qiagen, Germany Cat#74106 PrimeScriptTM RT reagent kit TakaRa, Japan Cat#TakaRa_RR037A SuperReal preMix plus SYBR Green Tiangen, Germany Cat#FP205-02 Dual-Luciferase assay system Promega, USA Cat#E1910 Mouse IL-9 DuoSet ELISA R&D system, USA Cat SYBR Green Assay:Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Aluminum hydroxide Sigma-Aldrich, USA Cat#239186 Antimycin A Sigma-Aldrich, USA Cat#A8674 Bovine serum albumin (BSA) Sigma-Aldrich, USA Cat#A7030 CaCl2 Merck, Germany Cat#C5670 Collagenase D Worthington, USA Cat#LS005273 CoCl2 Merck, Germany Cat#409332 Deoxyribonuclease I Beyotime Biotechnology, China Cat#D7076 DMEM Thermo Fisher Scientific, USA Cat#C11995500BT DMSO Sigma-Aldrich, USA Cat# D2650 Dooku1 MedChemExpress, USA Cat#HY-126010 Dimethyl malonate (DMM) Selleck, USA Cat#S6208 Fetal bovine serum (FBS) Every Green, Belgium Cat#11011-8611 Fibronectin Sigma-Aldrich, USA Cat#FG001 Fura-2 a.m. Sigma-Aldrich, USA Cat#F1201 Glucose Invitrogen, USA Cat#15023021 HEPES Invitrogen, USA Cat#15630130 Hoechest 33342 Beyotime Biotechnology, China Cat#C1029 KCl Thermo Fisher Scientific, USA Cat#ECCON84BT L-glutamine Sigma-Aldrich, USA Cat#G8540 Lipofectamine 3000 Thermo Fisher Scientific, USA Cat#L3000150 Methacholine Sigma-Aldrich, USA Cat#62-51-1 MgCl2 Invitrogen, USA Cat#AM9530G NaN3 Sigma-Aldrich, USA Cat#S2002 NaCl Invitrogen, USA Cat# AM9760G NaOH Merch, Germany Cat#1.09137 4% paraformaldehyde (PFA) Solarbio, China Cat#P1110 Oligomycin Sigma-Aldrich, USA Cat#495455 Ovalbumin (OVA) Sigma-Aldrich, USA Cat#S7951 Percoll GE Health, USA Cat#17-0891-01 Phorbol ester (PMA) Sigma-Aldrich, USA Cat#P8139 Protein A/G plus agarose beads Thermo Fisher Scientific, USA Cat#26159 RPMI1640 Thermo Fisher Scientific, USA Cat#C11875500BT Rotenone Sigma-Aldrich, USA Cat#R8875 Recombinant human IL-2 protein R&D system, USA Cat# BT-002 Recombinant human TGFb1 protein R&D system, USA Cat#7754-BH Recombinant human TGFb1 protein Peprotech, USA Cat#PHG9204 Recombinant mouse IL-4 protein R&D system, USA Cat#404-ML Recombinant mouse IL-4 protein PeproTech, USA Cat#214-14-5UG Recombinant mouse IL-6 protein R&D system, USA Cat#406-ML Recombinant mouse IL-12 protein Peprotech, USA Cat# 210–12 Recombinant mouse IL-23 protein R&D system, USA Cat#11269-ML SDS-PAGE sample loading buffer Beyotime Biotechnology, China Cat#P0015L Sodium pyruvate Sigma-Aldrich, USA Cat#S8636 TRI reagent Sigma-Aldrich, USA Cat#T9424 3-TYP MedChemExpress, USA Cat#HY-108331 Triton X-100 Sigma-Aldrich, USA Cat#S100PC Glucose Sigma-Aldrich, USA Cat#40163 (Continued on next page) 16 Cell Reports 44, 115136, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER GsMTx4 MedChemExpress, USA Cat#HY-P1410 Ionomycin Sigma-Aldrich, USA Cat#I0634 Yoda1 MedChemExpress, USA Cat#HY-18723 Critical commercial assays Co-IP buffer Thermo Fisher Scientific, USA Cat#88804 CD4+ T cell isolation kit Miltenyi Biotec, USA Cat#130-104-453 ECL kit Beyotime Biotechnology, China Cat#P10018AS Enhanced BCA protein assay kit Beyotime Biotechnology, China Cat#P0009 Foxp3/transcription factor staining buffer set BD Bioscience, USA Cat#560133 Mito stress test kit Seahorse Bioscience, USA Cat#101706-100 NE-PER nuclear and cytoplasmic extraction reagents Thermo Fisher Scientific, USA Cat#78835 RIPA lysis buffer Beyotime Biotechnology, China Cat#P0013B RNeasy mini kit Qiagen, Germany Cat#74106 PrimeScriptTM RT reagent kit TakaRa, Japan Cat#TakaRa_RR037A SuperReal preMix plus SYBR Green Tiangen, Germany Cat#FP205-02 Dual-Luciferase assay system Promega, USA Cat#E1910 Mouse IL-9 DuoSet ELISA R&D system, USA Cat Enzyme-linked Immunosorbent Assay:Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Aluminum hydroxide Sigma-Aldrich, USA Cat#239186 Antimycin A Sigma-Aldrich, USA Cat#A8674 Bovine serum albumin (BSA) Sigma-Aldrich, USA Cat#A7030 CaCl2 Merck, Germany Cat#C5670 Collagenase D Worthington, USA Cat#LS005273 CoCl2 Merck, Germany Cat#409332 Deoxyribonuclease I Beyotime Biotechnology, China Cat#D7076 DMEM Thermo Fisher Scientific, USA Cat#C11995500BT DMSO Sigma-Aldrich, USA Cat# D2650 Dooku1 MedChemExpress, USA Cat#HY-126010 Dimethyl malonate (DMM) Selleck, USA Cat#S6208 Fetal bovine serum (FBS) Every Green, Belgium Cat#11011-8611 Fibronectin Sigma-Aldrich, USA Cat#FG001 Fura-2 a.m. Sigma-Aldrich, USA Cat#F1201 Glucose Invitrogen, USA Cat#15023021 HEPES Invitrogen, USA Cat#15630130 Hoechest 33342 Beyotime Biotechnology, China Cat#C1029 KCl Thermo Fisher Scientific, USA Cat#ECCON84BT L-glutamine Sigma-Aldrich, USA Cat#G8540 Lipofectamine 3000 Thermo Fisher Scientific, USA Cat#L3000150 Methacholine Sigma-Aldrich, USA Cat#62-51-1 MgCl2 Invitrogen, USA Cat#AM9530G NaN3 Sigma-Aldrich, USA Cat#S2002 NaCl Invitrogen, USA Cat# AM9760G NaOH Merch, Germany Cat#1.09137 4% paraformaldehyde (PFA) Solarbio, China Cat#P1110 Oligomycin Sigma-Aldrich, USA Cat#495455 Ovalbumin (OVA) Sigma-Aldrich, USA Cat#S7951 Percoll GE Health, USA Cat#17-0891-01 Phorbol ester (PMA) Sigma-Aldrich, USA Cat#P8139 Protein A/G plus agarose beads Thermo Fisher Scientific, USA Cat#26159 RPMI1640 Thermo Fisher Scientific, USA Cat#C11875500BT Rotenone Sigma-Aldrich, USA Cat#R8875 Recombinant human IL-2 protein R&D system, USA Cat# BT-002 Recombinant human TGFb1 protein R&D system, USA Cat#7754-BH Recombinant human TGFb1 protein Peprotech, USA Cat#PHG9204 Recombinant mouse IL-4 protein R&D system, USA Cat#404-ML Recombinant mouse IL-4 protein PeproTech, USA Cat#214-14-5UG Recombinant mouse IL-6 protein R&D system, USA Cat#406-ML Recombinant mouse IL-12 protein Peprotech, USA Cat# 210–12 Recombinant mouse IL-23 protein R&D system, USA Cat#11269-ML SDS-PAGE sample loading buffer Beyotime Biotechnology, China Cat#P0015L Sodium pyruvate Sigma-Aldrich, USA Cat#S8636 TRI reagent Sigma-Aldrich, USA Cat#T9424 3-TYP MedChemExpress, USA Cat#HY-108331 Triton X-100 Sigma-Aldrich, USA Cat#S100PC Glucose Sigma-Aldrich, USA Cat#40163 (Continued on next page) 16 Cell Reports 44, 115136, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER GsMTx4 MedChemExpress, USA Cat#HY-P1410 Ionomycin Sigma-Aldrich, USA Cat#I0634 Yoda1 MedChemExpress, USA Cat#HY-18723 Critical commercial assays Co-IP buffer Thermo Fisher Scientific, USA Cat#88804 CD4+ T cell isolation kit Miltenyi Biotec, USA Cat#130-104-453 ECL kit Beyotime Biotechnology, China Cat#P10018AS Enhanced BCA protein assay kit Beyotime Biotechnology, China Cat#P0009 Foxp3/transcription factor staining buffer set BD Bioscience, USA Cat#560133 Mito stress test kit Seahorse Bioscience, USA Cat#101706-100 NE-PER nuclear and cytoplasmic extraction reagents Thermo Fisher Scientific, USA Cat#78835 RIPA lysis buffer Beyotime Biotechnology, China Cat#P0013B RNeasy mini kit Qiagen, Germany Cat#74106 PrimeScriptTM RT reagent kit TakaRa, Japan Cat#TakaRa_RR037A SuperReal preMix plus SYBR Green Tiangen, Germany Cat#FP205-02 Dual-Luciferase assay system Promega, USA Cat#E1910 Mouse IL-9 DuoSet ELISA R&D system, USA Cat Sequencing:Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Aluminum hydroxide Sigma-Aldrich, USA Cat#239186 Antimycin A Sigma-Aldrich, USA Cat#A8674 Bovine serum albumin (BSA) Sigma-Aldrich, USA Cat#A7030 CaCl2 Merck, Germany Cat#C5670 Collagenase D Worthington, USA Cat#LS005273 CoCl2 Merck, Germany Cat#409332 Deoxyribonuclease I Beyotime Biotechnology, China Cat#D7076 DMEM Thermo Fisher Scientific, USA Cat#C11995500BT DMSO Sigma-Aldrich, USA Cat# D2650 Dooku1 MedChemExpress, USA Cat#HY-126010 Dimethyl malonate (DMM) Selleck, USA Cat#S6208 Fetal bovine serum (FBS) Every Green, Belgium Cat#11011-8611 Fibronectin Sigma-Aldrich, USA Cat#FG001 Fura-2 a.m. Sigma-Aldrich, USA Cat#F1201 Glucose Invitrogen, USA Cat#15023021 HEPES Invitrogen, USA Cat#15630130 Hoechest 33342 Beyotime Biotechnology, China Cat#C1029 KCl Thermo Fisher Scientific, USA Cat#ECCON84BT L-glutamine Sigma-Aldrich, USA Cat#G8540 Lipofectamine 3000 Thermo Fisher Scientific, USA Cat#L3000150 Methacholine Sigma-Aldrich, USA Cat#62-51-1 MgCl2 Invitrogen, USA Cat#AM9530G NaN3 Sigma-Aldrich, USA Cat#S2002 NaCl Invitrogen, USA Cat# AM9760G NaOH Merch, Germany Cat#1.09137 4% paraformaldehyde (PFA) Solarbio, China Cat#P1110 Oligomycin Sigma-Aldrich, USA Cat#495455 Ovalbumin (OVA) Sigma-Aldrich, USA Cat#S7951 Percoll GE Health, USA Cat#17-0891-01 Phorbol ester (PMA) Sigma-Aldrich, USA Cat#P8139 Protein A/G plus agarose beads Thermo Fisher Scientific, USA Cat#26159 RPMI1640 Thermo Fisher Scientific, USA Cat#C11875500BT Rotenone Sigma-Aldrich, USA Cat#R8875 Recombinant human IL-2 protein R&D system, USA Cat# BT-002 Recombinant human TGFb1 protein R&D system, USA Cat#7754-BH Recombinant human TGFb1 protein Peprotech, USA Cat#PHG9204 Recombinant mouse IL-4 protein R&D system, USA Cat#404-ML Recombinant mouse IL-4 protein PeproTech, USA Cat#214-14-5UG Recombinant mouse IL-6 protein R&D system, USA Cat#406-ML Recombinant mouse IL-12 protein Peprotech, USA Cat# 210–12 Recombinant mouse IL-23 protein R&D system, USA Cat#11269-ML SDS-PAGE sample loading buffer Beyotime Biotechnology, China Cat#P0015L Sodium pyruvate Sigma-Aldrich, USA Cat#S8636 TRI reagent Sigma-Aldrich, USA Cat#T9424 3-TYP MedChemExpress, USA Cat#HY-108331 Triton X-100 Sigma-Aldrich, USA Cat#S100PC Glucose Sigma-Aldrich, USA Cat#40163 (Continued on next page) 16 Cell Reports 44, 115136, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER GsMTx4 MedChemExpress, USA Cat#HY-P1410 Ionomycin Sigma-Aldrich, USA Cat#I0634 Yoda1 MedChemExpress, USA Cat#HY-18723 Critical commercial assays Co-IP buffer Thermo Fisher Scientific, USA Cat#88804 CD4+ T cell isolation kit Miltenyi Biotec, USA Cat#130-104-453 ECL kit Beyotime Biotechnology, China Cat#P10018AS Enhanced BCA protein assay kit Beyotime Biotechnology, China Cat#P0009 Foxp3/transcription factor staining buffer set BD Bioscience, USA Cat#560133 Mito stress test kit Seahorse Bioscience, USA Cat#101706-100 NE-PER nuclear and cytoplasmic extraction reagents Thermo Fisher Scientific, USA Cat#78835 RIPA lysis buffer Beyotime Biotechnology, China Cat#P0013B RNeasy mini kit Qiagen, Germany Cat#74106 PrimeScriptTM RT reagent kit TakaRa, Japan Cat#TakaRa_RR037A SuperReal preMix plus SYBR Green Tiangen, Germany Cat#FP205-02 Dual-Luciferase assay system Promega, USA Cat#E1910 Mouse IL-9 DuoSet ELISA R&D system, USA Cat other:Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation Article Snippet: All animal experiments were performed with the approval of the Animal Ethics Committee of Academy of Military Medical Science and Beijing Normal University (IACUC-DWZX-2017-003 and CLS-EAW-2017-002; Beijing, China). |